Kvitova has been a fan favourite for many years thanks to her huge groundstrokes and booming left-handed serve.
There were fears her career would end prematurely after she was stabbed in her left hand by an intruder at her home in 2016.
The attack damaged tendons and nerves in her playing hand but she returned to playing in June 2017 and went on to reach the Australian Open final two years later.
In recent years she has fallen down the rankings and last made the quarter-finals of a Grand Slam in 2020.
Kvitova missed the entire 2024 season to have her son, Petr, and only returned to action in February.
It took her five tournaments to register her first victory – which came against Irina-Camelia Begu on clay in Rome in May – but that was her only win since becoming a mother.
Almost two months on from bidding a tearful goodbye to Wimbledon – which she called her “special place” – Kvitova called time on her career at the tournament where she twice reached the quarter-finals.
After quickly going down a break against world number 107 Parry, she rallied to reach 40-0 on serve then saved a break point to get her first game on the board.
But that proved to be her only success in a one-sided first set that Parry wrapped up in just 25 minutes.
Kvitova exited the court between sets to regroup – already visibly emotional at the prospect of this being her last match – but the second followed a similar pattern.
Winning just four points behind her own serve, Kvitova suffered a second-set bagel as Parry saw out a clinical victory.
“Since I wake up this morning, I felt it. I felt it would be not good,” Kvitova added.
“I couldn’t eat. I was really nervous. couldn’t move, I couldn’t swing, I couldn’t do anything.
“It was really difficult but I’m glad I did it.”